Journal:

Article Title: Molecular Characterization of Multidrug Resistance in Streptococcus mitis

doi:

Figure Lengend Snippet: Results of SmaI PFGE (A) and HinfI digestion of 250-bp gyrA PCR amplicons (B) of the 14 S. mitis isolates. Lanes 1 and 2, S. mitis strains isolated from the case patient in April and May 1996, respectively; lanes 3 through 14, 12 noncase S. mitis isolates. Lanes M, Lamba Ladder PFG marker (New England BioLabs, Beverly, Mass.) (A) and the 100-bp DNA ladder (Gibco BRL, Gaithersburg, Md.) (B).

Article Snippet: Lanes M, Lamba Ladder PFG marker (New England BioLabs, Beverly, Mass.) (A) and the 100-bp DNA ladder (Gibco BRL, Gaithersburg, Md.) (B). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Isolate MIC (μg/ml) a of: Molecular characterization by: CIP ERY CLI TET PEN CTX CRO IMP VAN PCR tet (M) d hybridization gyrA b mef c ermB c Case April 1996 64 >32 >16 >16 1 0.12 0.5 1 1 + − + + May 1996 64 >32 >16 >16 1 0.5 1 0.5 1 + − + + Noncase 1 0.5 >8 >8 ≤0.5 0.5 1 1 0.06 ≤0.5 − − + − 2 0.5 ≤0.5 ≤0.5 >4 0.06 0.06 0.06 0.06 ≤0.5 − − − + 3 2 ≤0.5 ≤0.5 >4 0.03 0.06 0.06 0.03 ≤0.5 − − − + 4 2 ≤0.5 ≤0.5 ≤0.5 0.5 0.25 0.25 0.06 ≤0.5 − − − − 5 2 >8 >8 ≤0.5 0.25 0.5 0.25 0.06 ≤0.5 − − + − 6 0.5 1 ≤0.5 ≤0.5 1 0.5 0.5 0.25 ≤0.5 − + − − 7 64 >8 >8 >4 0.03 0.06 0.06 0.06 ≤0.5 + − + + 8 0.5 2 ≤0.5 ≤0.5 0.03 0.06 0.06 0.03 ≤0.5 − + − − 9 4 >8 >8 >4 0.25 1 0.5 0.06 ≤0.5 − − + + 10 1 ≤0.5 ≤0.5 ≤0.5 0.03 ≤0.015 ≤0.015 0.03 ≤0.5 − − − − 11 0.5 >8 >8 >4 >8 >4 >4 2 ≤0.5 − − + + 12 0.5 2 ≤0.5 ≤0.5 4 2 2 1 ≤0.5 − + − − Open in a separate window a CIP, ciprofloxacin; ERY, erythromycin; CLI, clindamycin; TET, tetracycline; PEN, penicillin; CTX, cefotaxime; CRO, ceftriaxone; IMP, imipenem; VAN, vancomycin. b gyrA PCR amplicons (250 bp) were digested by Hin fI.

Techniques: Isolation, Marker